Optimum chance phylogenetic relationships of control area…

Optimum chance phylogenetic relationships of control area haplotypes produced from market purchased eyeball examples and sequences acquired from understood people.

Phylogenetic relationships were identical in Bayesian inference, maximum likelihood and maximum parsimony estimates (?lnL = 1399.28612; parsimony size = 141). Help values listed above nodes are Bayesian probabilities considering 5,000,000 resamples, maximum chance bootstrap help values according to 1,000 bootstrap replicates, and maximum parsimony bootstrap help values according to 2,000 bootstrap replicates. Help values are shown limited to clades of Sotalia guianensis and Sotalia fluviatilis haplotypes, and for the monophyly of Sotalia. Branch lengths are proportional to likelihood that is maximum divergence; scale shows portion of series divergence. Haplotypes 1–14, highlighted in bold, had been present in our study and correspond to GenBank accession #EU022531–EU022544.

To help expand confirm the relationships for the unknown sequences, we performed a nonphylogenetic cladistic analysis. We constructed a matrix of autapomorphies for Sotalia fluviatilis, Sotalia guianensis, Inia g. Geoffrensis, Inia g. Humboltiana, and Inia g. Boliviensis predicated on available series information in Genbank and our guide individuals, and completed a populace aggregation analysis ( Davis and Nixon 1992 ).

All eyeball?derived sequences decided by BLAST to be of cetacean origin provided species?specific character that is autapomorphic indicative of molecular positional homologies with Sotalia guianensis with no other types, unambiguously assigning all eyeballs of cetacean origin to Sotalia guianensis. The control area and Cytochrome b information sets have two and six diagnostic internet web internet sites, correspondingly, that assign all eyeball that is cetacean to Sotalia guianensis and unambiguously differentiate it from Sotalia fluviatilis and Inia spp. ( dining dining Table 1; online Appendix S1, S2).

Control area BEL MAO PVH
Variable position 39 136 139 160 175 231 256 269 270 362 381 387 404 463 504 559 Haplotype occurence
Inia spp. A T the G C G G/A A A T C C/T C/T C C/T A ? ? ?
Sotalia fluviatilis T T A G C/T G C/T T C C/T C T T C T A ? ? ?
Sotalia guianensis C G/T A/C A C/T G/A C/T C/T C/T C/T C/G C/T C/T C/T C/T C/A 22 11 1
CR_Hap01 C T A A T A C C C C C T C C T the 1 ? ?
CR_Hap02 C T A A T A C T C C C C T C T A 2 ? ?
CR_Hap03 C T A A T A C C C C C C T C T the 6 2 ?
CR_Hap04 C T A A T A C T C C C T C C T A 3 5 1
CR_Hap05 C T A A T A C T C T C T C C T the 1 2 ?
CR_Hap06 C T the A C the T T T C C T T C T the 1 ? ?
CR_Hap07 C T A A T A C T C C C C C C T A 3 curvy pussy? ?
CR_Hap08 C G C A T G C T C C C T C C T the 1 ? ?
CR_Hap09 C T A A T G C T C C C T C C T the 1 ? ?
CR_Hap10 C T A A T A C C C C C T T C T the 1 ? ?
CR_Hap11 C T A A T A C C C C C C T T T A ? 1 ?
CR_Hap12 C T A A T A C C C C C C T C T C ? 1 ?
CR_Hap13 C T the A C the C T T C C T T C C the 1 ? ?
CR_Hap14 C T A A T A C C C C G C T C T the 1 ? ?
Cytochrome b
adjustable position 49 78 124 297 367 396 462 501 528 531 561
Inia spp. G T A C G C T T A C T ? ? ?
Sotalia fluviatilis G C A G G C T the A C T ? ? ?
Sotalia guianensis G/A C/T G/A A G/A C/T C G C T C 16 7 1
CB_Hap01 G C A A G C C G C T C 8 4 1
CB_Hap02 A C A the G C C G C T C 1 ? ?
CB_Hap03 G T A A G C C G C T C 1 ? ?
CB_Hap04 G C G A G T C G C T C 3 1 ?
CB_Hap05 G C G A A T C G C T C 3 2 ?
  • Web web Sites highlighted in bold unambiguously assign the unknown eyeball samples to Sotalia guianensis. Those maybe maybe not highlighted differentiate noticed haplotypes. Control area haplotypes 1–14 match GenBank accession numbers EU022531?EU022544, while cytochrome b haplotypes 1–5 match GenBank accession numbers EU022545?EU022549. BEL = Belem, MAO = Manaus, PVH = Porto Velho.